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Sequencing

  • Perform sequencing using the recommended scripts in MinKNOW for the kit/barcode combination of your choice
  • Enable live-basecalling to monitor read quality and translocation speed
  • Speed/Quality drops (maybe due to many short reads)? Refuel flow cells as recommended by ONT
  • After run: Wash flowcells and re-use with other barcode set, adjusted starting voltage to account for voltage drift